ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader

Laura de Diego-García, Álvaro Sebastián-Serrano, Carolina Bianchi, Caterina Di Lauro, Miguel Díaz-Hernández

https://doi.org/10.1007/978-1-4939-9717-6_17

Imbalance in extracellular ATP levels in brain tissue has been suggested as a triggering factor for several neurological disorders. Here, we describe the most sensitive and reliable technique for monitoring the ATP levels in mice cerebrospinal samples collected by cisterna magna puncture technique and quantified using a microplate reader.

Skills

Posted on

04/10/2020